Rotary Evaporator — Standard Operating Procedure

1. Safety Precautions

• Use acid-resistant trap for TFA evaporation
• Ensure vacuum pump has adequate oil or dry pump is verified
• Secure all connections with clamps

2. Pre-Operation

• [ ] Check receiving flask is empty and clean
• [ ] Verify bath fluid level and temperature set point
• [ ] Check condenser coolant flow
• [ ] Ensure bump trap is in place

3. Operation

1. Fill flask to ≤50% of nominal volume
2. Attach flask to bump trap with clip
3. Lower flask into bath
4. Start rotation at 100 – 150 rpm
5. Apply vacuum gradually
6. Monitor for bumping
7. When evaporation complete, release vacuum
8. Remove flask

4. Cleaning

Step	Solvent	Method
Rinse	Methanol	Swirl and drain
Rinse	DI water	Swirl and drain
Drying	Acetone	Rinse, air dry