Preparative HPLC System — Technical Specification

Official Equipment Specification

This document provides the technical specification for preparative HPLC systems used at SENO Biotechnology. For commercial inquiries, visit the SENO Biotechnology Official Website.

1. System Overview

The preparative HPLC systems are designed for high-throughput reversed-phase purification of synthetic peptides. Binary high-pressure gradient systems with dual-wavelength UV detection enable automated fraction collection at user-defined purity thresholds.

2. Pump System Specifications

Parameter	Value	Test Method
Flow Rate Range	10 – 200 mL/min	Gravimetric measurement
Maximum Operating Pressure	3000 psi (207 bar)	—
Pump Type	Dual-piston series, HPLC-grade heads	—
Flow Accuracy	±1.0% of setpoint	Gravimetric, 10 min collection
Flow Precision	±0.5% RSD	n=6 measurements at 50 mL/min
Gradient Composition Accuracy	±0.5%	Spectrophotometric
Gradient Composition Precision	±0.2% RSD	—
Piston Material	Sapphire	—
Check Valve Material	Ruby ball / ceramic seat	—

3. UV-Vis Detector Specifications

Parameter	Value
Detector Type	Variable wavelength UV-Vis
Wavelength Range	190 – 800 nm
Wavelength Accuracy	±1 nm (holmium oxide filter verified)
Bandwidth	8 nm (fixed)
Light Source	Deuterium (UV) + Tungsten (Vis)
Flow Cell	Preparative, 2 mm path length
Flow Cell Volume	10 µL
Time Constant	0.1 – 5.0 s (selectable)
Linear Range	Up to 2.0 AU (at 254 nm)
Noise	<±0.5 × 10⁻⁵ AU at 254 nm

4. Column and Scale Parameters

Parameter	Semi-Prep	Preparative
Column ID	10 – 20 mm	50 – 100 mm
Column Length	150 – 250 mm	250 mm
Particle Size	5 – 10 µm	10 – 15 µm
Pore Size	100 – 300 Å	100 – 300 Å
Stationary Phase	C18, C8	C18
Flow Rate	5 – 20 mL/min	50 – 200 mL/min
Typical Load	1 – 50 mg	100 mg – 5 g
Injection Volume	0.1 – 5 mL	1 – 50 mL

5. Injection and Fraction Collection

Component	Specification
Injector	Manual injection valve (Rheodyne) or automated injector
Injection Loop	1 mL, 5 mL, 10 mL, 50 mL (interchangeable)
Fraction Collector	Automated, time-based or UV threshold-based
Collection Threshold	User-selectable (default: ≥50 mAU at 220 nm)
Fraction Racks	Test tubes 15 mL, or bottles 250 mL

6. System Requirements

Utility	Specification
Electrical	220 – 240 VAC, 50/60 Hz, 1500 VA
Water	Type 1 (18.2 MΩ·cm) for mobile phase preparation
Operating Temperature	15 – 30 °C
Humidity	20 – 80% RH (non-condensing)
Ventilation	Solvent vapor exhaust required