Analytical HPLC System — Technical Specification

Official Equipment Specification

This document provides the technical specification for analytical HPLC systems used at SENO Biotechnology. For commercial inquiries, visit the SENO Biotechnology Official Website.

1. Technical Parameters

Parameter	Specification
Flow Rate Range	0.1 – 5.0 mL/min
Maximum Operating Pressure	6000 psi (414 bar)
Solvent Delivery	Quaternary or binary gradient
Degasser	Online vacuum degassing (4 channels)
Injector	Autosampler with sample cooling (4 – 40 °C)
Injection Volume	0.1 – 100 µL
Column Compartment	Peltier-controlled, 10 – 85 °C

2. Detector Specifications

Parameter	DAD (Diode Array)	Specification
Wavelength Range	190 – 800 nm	Full spectrum
Lamp	Deuterium + Tungsten	Automatic switch
Bandwidth	1 – 40 nm	Adjustable
Data Rate	Up to 100 Hz	Real-time spectral acquisition
Noise	<±0.5 × 10⁻⁵ AU	At 254 nm

3. System Performance

Parameter	Specification	Test Method
Flow Precision	<0.3% RSD	Retention time repeatability
Injection Precision	<0.5% RSD	Area repeatability (n=6)
Gradient Accuracy	±0.5%	Composition verification
Wavelength Accuracy	±1 nm	Holmium oxide filter
Sensitivity (S/N)	>1000	1 µL of 1 µg/mL caffeine

4. Column Specifications

Parameter	Typical Value
Column Dimensions	150 × 4.6 mm, 250 × 4.6 mm
Particle Size	3 – 5 µm
Pore Size	100 – 300 Å
Stationary Phase	C18, C8, C4, HILIC