LC-MS System — Technical Specification

1. Mass Spectrometer Specifications

Parameter	Specification
Mass Range (m/z)	50 – 3000
Resolution	≥10,000 (FWHM at m/z 1000)
Mass Accuracy	<5 ppm RMS (external calibration); <2 ppm (external with lock mass)
Mass Stability	±0.1 Da over 24 h (ambient conditions ±2 °C)
Scan Speed	≤10,000 Da/s
Dynamic Range	4 × 10⁴ (4 orders of magnitude)
Detector	Microchannel plate (MCP) or electron multiplier

2. ESI Source Parameters

Parameter	Positive Mode	Negative Mode
Capillary Voltage	3.5 – 4.5 kV	2.5 – 3.5 kV
Cone Voltage	20 – 60 V	20 – 60 V
Source Temperature	100 – 150 °C	100 – 150 °C
Desolvation Temperature	250 – 350 °C	250 – 350 °C
Desolvation Gas (N₂)	500 – 800 L/h	500 – 800 L/h
Cone Gas (N₂)	50 – 100 L/h	50 – 100 L/h
Nebulizer Gas	N₂, 7 bar	N₂, 7 bar

3. LC Component Specifications

Parameter	Specification
Flow Rate	0.1 – 2.0 mL/min (splitting to MS)
Max Pressure	6000 psi
Solvent Delivery	Binary or quaternary
Detector (inline)	DAD or UV (optional, for LC-MS)
Column	C18, 2.1 × 50 – 100 mm, 1.7 – 3.5 µm

4. System Performance

Test	Parameter	Acceptance
Sensitivity (ESI+)	1 pg reserpine (609 m/z)	S/N >100:1
Mass Accuracy	External calibration	<5 ppm over full range
Isotopic Distribution	Leucine enkephalin (556 m/z)	>95% match to theoretical